Product Info Summary
SKU: | M03245-2 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ACAT2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03245-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-ACAT2 Rabbit Monoclonal Antibody catalog # M03245-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACAT2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03245-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27A26
Isotype
IgG
Immunogen
A synthesized peptide derived from human ACAT2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03245-2 is reactive to ACAT2 in Human, Mouse, Rat
Applications
M03245-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
41 kDa
Calculated molecular weight
41.351kDa
Background of ACAT2
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ACAT2 using anti-ACAT2 antibody (M03245-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT2 antigen affinity purified monoclonal antibody (Catalog # M03245-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ACAT2 (Source: Uniprot.org, NCBI)
Gene Name
ACAT2
Full Name
Acetyl-CoA acetyltransferase, cytosolic
Weight
41.351kDa
Superfamily
thiolase-like superfamily
Alternative Names
acetoacetyl Coenzyme A thiolase; acetyl-CoA acetyltransferase 2; acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase); acetyl-Coenzyme A acetyltransferase 2; ACTL; Cytosolic acetoacetyl-CoA thiolase; EC 2.3.1; EC 2.3.1.9 ACAT2 acetyl-CoA acetyltransferase 2 acetyl-CoA acetyltransferase, cytosolic|acetoacetyl Coenzyme A thiolase|acetyl-CoA transferase-like protein|cytosolic acetoacetyl-CoA thiolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACAT2, check out the ACAT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACAT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACAT2 Rabbit Monoclonal Antibody (M03245-2)
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